home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410p.zip
/
M94A3236.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
3KB
|
42 lines
Document 3236
DOCN M94A3236
TI Induction of demyelination and neuronal death by HIV-1 gp120 depends on
neural development.
DT 9412
AU Kimura-Kuroda J; Nagashima K; Yasui K; Tokyo Metro. Inst. for Neurosci.,
Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):122 (abstract no. PA0107). Unique
Identifier : AIDSLINE ICA10/94369339
AB OBJECTIVE: In HIV encephalopathy, diffuse demyelination and neuronal
loss have been observed frequently, while the pathogenesis remains
obscure. Although the neurotoxicity of gp120 as a causative factor has
been reported, it is still not recognized. To clarify the neurotoxicity
of gp120, its effects on neural cells were examined using rat primary
culture of cerebral cortex at different developmental stages. METHODS:
Cerebral cortex culture was prepared from 18 day embryonic rats. Detail
was described in our previous paper (Kimura-Kuroda et al, 1994, Arch.
Virol. in press). RESULTS: In immature neural cells, (1-3 days in
culture), gp120 bound to most neurons and the addition of gp120 caused
approximately 50% of neuronal death. The toxicity of gp120 against
neurons was confirmed its own effect but not due to the contamination of
endotoxin or mycoplasma. While in mature neural cells (4-7 weeks in
culture), addition of gp120 did not show any toxicity against neurons,
but it resulted in significant inhibition of myelin formation. By the
addition of gp120, the number of oligodendrocyte was not different but
their processes were reduced in length and arborization. This indicates
that gp120 might cause a functional disorder of oligodendrocyte.
DISCUSSION & CONCLUSION: Gp120 might have at least two separate effects
on oligodendrocytes and neurons under certain developmental conditions.
The effects of HIV-1 gp120 can explain both neuronal loss and diffuse
demyelination in HIV encephalopathy.
DE Animal Cell Death/*DRUG EFFECTS Cells, Cultured Cerebral
Cortex/*CYTOLOGY Comparative Study Embryo HIV Envelope Protein
gp120/*TOXICITY HIV-1/*PHYSIOLOGY Myelin Sheath/*DRUG
EFFECTS/PHYSIOLOGY Neurons/*CYTOLOGY/DRUG EFFECTS/PATHOLOGY
Oligodendroglia/*CYTOLOGY/DRUG EFFECTS Rats Time Factors MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).